Background: Probiotics are defined as different microorganisms that may have positive effects on preventing or treatment of special pathologic conditions. Lactobacillus casei and L. paracasei as probiotics could induce the apoptosis in human cancer cells in vitro. Chronic myeloid leukemia is categorized as a blood cells cancer and the most common type of leukemia. The expression of mutant p53 protein increases resistance against apoptosis in cancer lines. So the aim of this study was to evaluate the effect of L. casei and L. paracasei cell wall and cytoplasmic fractions on the expression of mutant p53 protein in K562 cancer line by ELISA.Materials and Methods: First, L. casei and L. paracasei was cultured and disrupted by sonication and finally cell wall separated from cytoplasmic extraction by centrifugation, then prepared different concentrations of cell wall and cytoplasmic fraction. Finally, p53 protein levels were measured by ELISA, in cells treated with the cell wall and cytoplasmic fraction.Results: ELISA result showed that cell wall and cytoplasmic fraction statistically (P˂0.05) reduced the levels of mutant p53 protein compared to the control.Conclusion: It’s concluded that there is a relationship between reduced expression of p53 protein and induction of apoptosis in cells with mutant p53 gene. It could provide a way for cancer treatment in a smart manner.

Purpose: To illustrate the regression of a metastatic lesion through ophthalmic imaging and correlating findings with standard chest imaging and treatment with osimertinib, an oral chemotherapy agent specific to Epidermal Growth Factor Receptor + Non-small Cell Lung Cancer (EGFR+ NSCLC). Case Report: A 63-year-old Asian male presented to ophthalmology with a complaint of left blurry vision. Initial ophthalmic exam revealed a choroidal lesion and imaging results highlighted a spiculated lung mass with brain and bony metastases. Osimertinib was chosen for its specificity and ability to cross the blood–, brain barrier. Follow-up ophthalmic and radiographic imaging were repeated over the course of treatment. Conclusion: After the initiation of osimertinib, ophthalmic and computed tomography imaging highlighted the regression of the ocular metastatic disease and primary malignancy, respectively. Osimertinib is an effective first-line treatment of EGFR+ NSCLC and corresponding metastatic sites. Additionally, ophthalmic imaging can be used to monitor general response to chemotherapy agents when ocular metastasis is identified.

Introduction & objective: The third common primary intracranial tumors are pituitary adenomas. Atypical pituitary tumors are characterized by MIB-1>3% & significant P53 nuclear staining. The aim of this study is to evaluate the relationship of the mutant P-53 & MIB-1 level with the outcome of these tumors.Materials & Methods: This Cohort study is conducted in both retrospective & prospective phases from April 2013 to October 2015, 83 patients were enrolled, and inclusion criteria were presence of radiological, clinical or endocrinologic evidence of functional or non-functional pituitary adenomas. Besides, all the patients underwent surgery, whether trans-sphenoidal or trans-cranial, for evacuation of adenomas. Follow-up radiologic and clinical evaluation were done 6 weeks, 6months, and one year after the surgery in all the cases.Results: The non-functional tumors were significantly more aggressive (82.2% versus 55.3% in functional adenomas) (P=0.008). No significant relationship was found between outcome MIB-1 & P53 levels (P=0.49, P=0.834 respectively). On the other hand, invasiveness was significantly related to outcome (39 patients (84.7%) with unaccepted outcome and 19 (51.3%) cases with accepted outcome were invasive, P=0.001).Conclusions: Among invasiveness & MIB-1 & P-53 level, the role reliable prognostic factors for anticipating the outcome seems to be invasiveness and the other factors can be just assumed as confounder factors.

With the aim of comparing the grain yield and yield components of 16 oilseed rape (Brassica napus L. ) mutant lines with cultivars Talayeh, Zarfam, Express and Okapi as check cultivar an experiment was conducted based on randomized complete block design with three replications during 2015-2016 cropping season in experimental field of Seed and Plant Improvement Institute (SPII) in Karaj. Analysis of variance showed significant differences among the genotypes in length of vegetation period, seed and oil yield. The results showed that the mutant line Z-800-6 had the most grain and oil yield compared to Okapi. The maximum and minimum number of days to maturity was related to mutant lines T-800-6 (256 days) and Exp-900-1 (238 days), respectively. Principal component analysis indicated that three principal components explained more than 79 percent of the variation and were correlated with grain yield, flowering time and oil yield respectively. Stepwise regression analysis showed that number of pod per plant and per main stem, pod length on main stem and 1000-grain weight explained the most of the variation of yield suggesting that they can be used as indices for increasing grain and oil yield in oilseed rape breeding.

Background: IL-6 plays an important role in cancer metastasis. Therefore, it seems that interleukin-6 inhibition can act as an anticancer agent.Objectives: In this study, the anti-proliferative and IL-6 signal transduction inhibitory effects of a mutant IL-6 receptor (mIL-6R), were examined on an ovarian cancer cell line.Methods: The intein1-mIL-6R was expressed in E. coli BL21 at various inducer concentrations and temperatures. The expressed protein was purified using the IMPACT system, and the best examined condition (temperature, time, cleavage buffer pH) for intein1auto-cleavage was achieved. The anti-proliferative effects of mIL-6R on OVCAR-3 cancer cells was investigated using MTT assay and RT-PCR to determine its effects on the suppression of the JAK-STAT pathway genes.Results: The most soluble mIL-6R was obtained at 20 °C, 0.5 mM IPTG. The highest level of intein1 cleavage occurred at 25 °C, 24 hours of incubation, and pH 4 of cleavage buffer. mIL-6R diminished the survival of OVCAR-3 cells in comparison to PBS (p value = 0.024), with 48 hours IC50 of 1.117 μg/mL. Also, mIL-6R significantly reduced the expression of the JAK, STAT, and VEGF genes.Conclusion: mIL-6R can be further evaluated for its potential activity against disorders associated with the increase of IL-6.